Santa Cruz Biotechnology, Inc.
SANTA CRUZ
NKCC1 (N-16):
sc-21545
BIOTECHNOLOGY
BACKGROUND
Na-K-Cl cotransporters (NKCC) are channel proteins that aid in
the transcellular movement of chloride across both secretory and
absorptive epithelia (1,2). The 170 kDa NKCC1 is expressed in
muscle cells, neurons, and red blood cells (1-3). In the basolateral
membrane of secretory epithelia, NKCC1 mediates active chloride
secretion (3). The gene encoding human NKCC1 maps to
chromosome 5q23.3 (2). In mice, disruption of the NKCC1 gene
leads to deafness and impaired balance (4). NKCC2 is specifically
expressed in the kidney where it mediates active reabsorption of
sodium chloride in the thick ascending limb of the loop of
Henle (3). NKCC2 is sensitive to the clinically important diuretics
furosemide and bumetanide (3). The gene encoding human
NKCC2 maps to chromosome 15q15-q21 and mutations in this
gene lead to Bartter鈥檚 syndrome, an inherited hypokalaemic
alkalosis (3,5). NCCT is a thiazide-sensitive Na-Cl cotransporter
that is primarily expressed in the distal convoluted tubule of the
kidney where it accounts for a significant fraction of net renal
sodium reabsorption (5). The gene for human NCCT map to
chromosome 16q13 (6). Mutations in the gene encoding NCCT
cause Gitelman鈥檚 syndrome, a subset of Bartter鈥檚 syndrome (5,7).
SOURCE
NKCC1 (N-16) is an affinity purified goat polyclonal antibody
raised against a peptide mapping near the amino terminus of
NKCC1 of human origin.
PRODUCT
Each vial contains 200 碌g IgG in 1.0 ml of PBS containing
0.1% sodium azide and 0.2% gelatin.
Blocking peptide is available for competition studies (sc-21545 P)
(100 碌g peptide in 0.5 ml PBS with 0.1% sodium azide and
100 碌g BSA).
SPECIFICITY
NKCC1 (N-16) is recommended for the detection of NKCC1 of
mouse, rat and human origin by Western blotting and ELISA.
Recommended dilution range for Western blot analysis:
1:100鈥?:1000. Recommended starting dilution: 1:100.
STORAGE
Store at 4擄 C, do not freeze; stable for one year from the date of
shipment.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
REFERENCES
1. Xu, J.C., Lytle, C., Zhu, T.T., Payne, J.A., Benz, E., Jr., and
Forbush, B., III 1994. Molecular cloning and functional expression
of the bumetanide-sensitive Na-K-Cl cotransporter. Proc. Natl.
Acad. Sci. USA 91: 2201-2205.
2. Payne, J.A., Xu, J.C., Haas, M., Lytle, C.Y., Ward, D., and
Forbush, B., III 1995. Primary structure, functional expression,
and chromosomal localization of the bumetanide-sensitive
Na-K-Cl cotransporter in human colon. J. Biol. Chem.
270: 17977-17985.
3. Quaggin, S.E., Payne, J.A., Forbush, B., III, and Igarashi, P.
1995. Localization of the renal Na-K-Cl cotransporter
gene (Slc12a1) on mouse chromosome 2. Mamm. Genome
6: 557-558.
4. Delpire, E., Lu, J., England, R., Dull, C., and Thorne, T. 1999.
Deafness and imbalance associated with inactivation of the
secretory Na-K-2Cl co-transporter. Nat. Genet. 22: 192-195.
5. Simon, D.B., Nelson-Williams, C., Bia, M.J., Ellison, D.,
Karet, F.E., Molina, A.M., Vaara, I., Iwata, F., Cushner, H.M.,
Koolen, M., Gainza, F.J., Gitleman, H.J., and Lifton, R.P. 1996.
Gitelman's variant of Bartter's syndrome, inherited hypokalaemic
alkalosis, is caused by mutations in the thiazide-sensitive Na-Cl
cotransporter. Nat. Genet. 12: 24-30.
6. Mastroianni, N., De Fusco, M., Zollo, M., Arrigo, G.,
Zuffardi, O., Bettinelli, A., Ballabio, A., and Casari, G. 1996.
Molecular cloning, expression pattern, and chromosomal
localization of the human Na-Cl thiazide-sensitive
cotransporter (SLC12A3). Genomics 35: 486-493.
7. Mastroianni, N., Bettinelli, A., Bianchetti, M., Colussi, G.,
De Fusco, M., Sereni, F., Ballabio, A., and Casari, G. 1996. Novel
molecular variants of the Na-Cl cotransporter gene are responsible
for Gitelman syndrome. Am. J. Hum. Genet. 59: 1019-1026.
U.S. 1.800.457.3801 鈥?831.457.3800 鈥?fax 831.457.3801 鈥?Europe + 800 4573 8000 鈥?49 (0)6221 4503 0 鈥?fax 49 (0)6221 4503 45 鈥?www.scbt.com
April 2001
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